A set of ahead (5-AAGGGTTACTTGATTAGCTGTGTTTGG-3) and reverse (5-GGCAGACACGTACGGGGGC-3) primers that incorporated BL-21 (GE Healthcare Existence Sciences) were transformed with the (LA)2-LAPTM4B/pGEX-2?T construct, and manifestation of recombinant (LA)2-LAPTM4B/GST fusion protein was induced with 0
A set of ahead (5-AAGGGTTACTTGATTAGCTGTGTTTGG-3) and reverse (5-GGCAGACACGTACGGGGGC-3) primers that incorporated BL-21 (GE Healthcare Existence Sciences) were transformed with the (LA)2-LAPTM4B/pGEX-2?T construct, and manifestation of recombinant (LA)2-LAPTM4B/GST fusion protein was…